Frequency of ABO, Subgroup ABO and Rh(D)


By Fathelrahman Mahdi Hassan.


Background: There are differences in the distribution of ABO, subgroup ABO and Rh(D) blood groups in different populations of the world. Relatively little information is available about blood group distributions in Sudanese population. Objectives: To see the frequency of ABO, subgroup ABO and Rh(D) blood groups in major Sudanese ethnic groups(Danagla, Shaygia and Gaaleen). Subjects and Methods: Blood testing for ABO, subgroup ABO and Rh (D) typing was done over six months, in 300 unrelated individuals, from both genders. Blood samples were collected from students of the college of medical laboratory science – Sudan University of Science and Technology using finger prick method and following routine slide method. Results: Blood group "O" was the most predominant (52.7%) in both Rh positive and negative subjects, followed by blood group A, B and AB. Majority (98.0%) of the subjects were Rh (D) positive and only 2% were Rh negative. The predominant subgroup of ABO was A2 (14.1%). Conclusions: The frequency of ABO blood groups in both Rh positive and negative subjects among the major Sudanese ethnic group was similar to that reported from neighbouring regions. Key words: ABO, subgroup ABO, Rh(D), blood groups.


  • Garratty G, Dzik W, Issitt PD, Lublin DM, Reid ME, Zelinski T. Terminology for blood group antigens and genes – historical origins and guideline in the new millennium. Transfusion 2000; 40:477-89.
  • Mollison PL. The genetic basis of the Rh blood group system. Transfusion 1994; 34:539-41.
  • John DR. Technical Manual of American Association of Blood Banks. In: Blood group and genetics. 12 ed. USA: American Association of Blood Banks; 1996; p.173-261.
  • Jolly JG. Medicolegal significance of human blood groups. J Indian Med Assoc. 2000; 98:340-1.
  • Khurshid B, Naz M, Hassan M, Mabood SF. Frequency of ABO and Rh (D) blood groups in district Swabi, NWFP, Pakistan. J Sci Tech University Peshawar 1992;16:5-6.
  • Shayeh A, Feudy AH. Distribution of ABO blood groups and the Rh factor in Palestinian living in Northern part of the West Bank. Najah Res J. 1988;2(1):35-41.
  • Bashwari LA, Al-Mulhim AA, Ahmad MS, Ahmed MA. Frequency of ABO blood groups in Eastern Region of Saudi Arabia. Saudi Med J 2001;22:1008-12.
  • Younis A.S, Amany S.A., Alaa S.S., Basma B. H. Frequency of ABO and Rh(D) blood groups in five governorates in Gaza-StripPak J Med Sci 2007 ; 23 ,(6)924-7.
  • Khalil IA, Phrykian S, Farr AD. Blood group distribution in Sudan. Gene Geogr 1989;3:7-10.
  • Ahmed R, Mohammed U. ABO blood group distribution among Saudi citizens related to their regional or original tribal location. Kuwait J Sci Eng 2002; 19: (1): 75-81.
  • Al-Bustan S, El-Zawahri M, Al-Azmi D, Al-Bashir AA. Allele frequencies and molecular genotyping of the ABO blood group system in a Kuwaiti population. Int J Hematol 2002;75:147-53.
  • 13. Sakharov RS, Nofal’Kh K. The frequency of ABO blood groups and the expression of group antigens and isohemagglutinins in Syrian Arabs. Sud Med Ekspert 1996;39:34-6.
  • Nasif E. The incidence of blood groups in Lebanese. Lebanon Med J 1953;6:346-9. 15. Dean L .The ABO blood group.". Blood Groups and Red Cell Antigens. (2005"Chapter 5). Available from: antigen.chapter.ch05ABO
  • Hanania S, Hassawi D, Irshaid N. Allele frequency and molecular genotypes of ABO blood group system in Jordanian population. J Med Sci 2007;7:51-8.
  • Ami-ud-Din M, Fazeli N, Rafiq MA, Malik S. Serological study among the municipal employees of Tehran, Iran. Distribution of ABO and Rh blood groups. Hematology 2004;7:502-4.
  • Skaik Y, El-Zyan N. Spectrum of ABO and Rh(D) blood groups amongst the Palestinian students at Al-Azhar University-Gaza. Pak J Med Sci 2006;22:333-5.
  • Mollison PL, Engelfriet CP, Conteras M: ABO, Lewis Ii and P Groups. In: Mollison PL, Engelfriet CP, Conteras M, editors. Blood transfusion in clinical medicine. 9th ed. Oxford: Blackwell Scientific Publications; 1993; p.4,150-1.
  • Abo Algasim EI, Malik H, Tarq E. Dawoud equencies of ABO, Rh-D and Kell (Kpa,Kpb) Blood Group Antigens in Dinka Sudanese Ethnic Group, SUST, 2007; 01-12.
  • Contemporary views of human variation. In: William A, Harry N, Robert J, Lynn K. Understanding physical anthropology and archeology, 1998;112.